Degenerate primer design to clone the human repertoire of immunoglobulin heavy chain variable regions
نویسندگان
چکیده
منابع مشابه
Immunoglobulin heavy chain constant regions regulate immunity and tolerance to idiotypes of antibody variable regions.
Particular syngeneic adjuvant-free monoclonal antibodies are immunogenic and elicit antibody responses against the variable region idiotypes (Ids). We here study how heavy-chain constant regions (C(H)) regulate immune responses to Ids of free, uncomplexed monoclonal antibodies. To this end, we selected two hybridomas, called Id(3) and Id(A.01), that produce immunogenic IgM(lambda)2 directed tow...
متن کاملImmunoglobulin heavy chain gene organization in mice: analysis of a myeloma genomic clone containing variable and alpha constant regions.
We have isolated a myeloma genomic DNA clone containing the variable and constant regions of a mouse alpha chain. Restriction enzyme analyses and electron microscopic R loop mapping have demonstrated that the variable region is separated from the constant region by 6.8 kilobases of intervening DNA. In addition, two intervening DNA sequences of 100--200 bases separate the constant region into th...
متن کاملThe 48-base-long primordial building block of immunoglobulin light-chain variable regions is complementary to the primordial building block of heavy-chain variable regions.
The ancestral gene for immunoglobulin light-chain variable regions (Ig VLs) of the kappa as well as the lambda class apparently arose from about 12 tandem repeats of the 48-base-long primordial building block sequence TCT-TGC-GCA-GTA-AGT-CCA-CTC-CAG-GTC-ATA-TCC-AGT-CAG-GCT-GCT-GAA. Even today, amino acid residues 67 to 82 of each Ig V kappa L are still specified by a direct descendant in toto o...
متن کاملHigh-Throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire
Currently existing immune repertoire sequencing technologies yield data on only one of the two chains of immune receptors [1–3]. Sequence analysis of VH:VL pairs is therefore currently performed by microtiter-well sorting of individual B cells followed by single-cell RT-PCR (scRT-PCR) and Sanger sequencing [3–10]; however at most a few hundred VH:VL pairs (a number dwarfed by the enormous size ...
متن کاملHigh-Throughput Sequencing of the Paired Human Immunoglobulin Heavy and Light Chain Repertoire
Currently existing immune repertoire sequencing technologies yield data on only one of the two chains of immune receptors [1–3]. Sequence analysis of VH:VL pairs is therefore currently performed by microtiter-well sorting of individual B cells followed by single-cell RT-PCR (scRT-PCR) and Sanger sequencing [3–10]; however at most a few hundred VH:VL pairs (a number dwarfed by the enormous size ...
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ژورنال
عنوان ژورنال: World Journal of Microbiology and Biotechnology
سال: 2011
ISSN: 0959-3993,1573-0972
DOI: 10.1007/s11274-011-0830-3